Maintaining balance in your cell cultures

Balanced salt solutions provide an environment that helps lớn maintain the structural and physiological integrity of cellsin vitro.Trust Gibteo Balanced Salternative text Solutions to lớn provide an isotonic buffer system that keeps your cells in the physiological pH range và helps to maintain high viability during short-term incubations.

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Available in many formulations & formats, both liquid & powder. All powdered balanced salt solutions are produced without sodium bicarbonate khổng lồ increase stability. Powder formats will require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see thisprotocolfor details).

All Gibteo Balanced Salternative text Solutions are manufactured in state-of-the-art cGMP, ISO-certified facilities lớn ensure the highest quality and consistency for reproducible results every day. Tests include osmolality, pH, stability, the absence of bacterial and fungal contamination, and endotoxin tests.

Shop all balanced salternative text solutions Compare PBS & DPBS Compare HBSS và EBSS


The essential functions of a balanced salternative text solution are khổng lồ maintain pH & osmotic balance as well as provide your cells with water và essential inorganic ions. Gibco Balanced Salternative text Solutions are available in many different formulations and formats. To help you choose which balanced salternative text solution will meet your specific retìm kiếm needs, use the following selection guides lớn compare PBS, DPBS, HBSS, và EBSS.

Here are the comtháng formulation components found in balanced salt solutions và how they may impact your cell culture.


Calcium (Ca2+) và magnesium (Mg2+)are involved in cell adhesion, so it is recommended that calcium and magnesium are present when cells need khổng lồ remain attached lớn the culture substrate. Formulations with or without calcium và magnesium can be found in PBS, DPBS, HBSS, & EBSS.


Glucose và pyruvateact as energy sources và tư vấn cell survival. Their inclusion in a balanced salt solution may help lớn keep cells alive sầu longer in the absence of culture medium. Formulations with or without glucose và pyruvate can be found in PBS, DPBS, HBSS, and EBSS.


Phenol redis a pH indicator that exhibits a gradual color change from yellow khổng lồ pink between pH 6.8 và 8.2. Formulations with or without phenol red can be found in HBSS and EBSS.


Sodium bicarbonatehelps lớn stabilize pH. The amount of sodium bicarbonate in EBSS is designed for use under 5% CO2 but that in HBSS is much lower and for use out of a CO2 incubator. Formulations with or without sodium bicarbonate can be found in HBSS & EBSS.

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Phosphate buffered saline (PBS) and Dulbecco’s phosphate buffered saline (DPBS) are commonly used in biological applications. Both include sodium chloride và phosphate buffer and are formulated khổng lồ prevent osmotic shoông chồng and maintain water balance in living cells.

PBS is a commonly used buffer with a simple formulation, while DPBS also includes potassium chloride and is available in a larger variety of formulations, including with or without calcium and magnesium và with or without glucose và pyruvate.

Your choice of an appropriate buffer will depover on your application of interest. For example, khổng lồ wash cells before dissociation, buffer without calcium/magnesium is preferred; lớn keep cells viable for moderate periods of time outside of growth medium, buffer with glucose/pyruvate & calcium/magnesium is suitable.


Application Ca2+, Mg2+ Glucose, pyruvate PBS Cat. No. DPBS Cat. No. Publications
Cell dissociation, immunostaining
Tissue dissection, assay, immunostaining, perfusion + +
Immunoprecipitation, immunohistochemistry +
Cell therapy applications
+
Flow cytometry

Hank’s balanced salternative text solution (HBSS) và Earl’s balanced salternative text solution (EBSS) are isotonic solutions used lớn maintain osmolality and pH in biological applications. Both HBSS & EBSS include glucose và sodium bicarbonate for short-term maintenance of cells outside of growth medium. The amount of sodium bicarbonate in EBSS is designed khổng lồ maintain pH under 5% CO2, while HBSS has lower sodium bicarbonate levels và is intended for incubation without CO2. These buffers also come in a variety of formulations, including with or without calcium/magnesium & with or without phenol red, so you may choose the appropriate buffer depending on your application.


Application Ca2+,Mg2+ Glucose Phenol red Sodium bicarbonate HBSS Cat.No. EBSS Cat.No. Publications
Tissue dissociation, perfusion + + 14175 14155
Dissection, staining + 10X: 14185
Dissection, cell washing + + + 14170
Live cell procedure, microscopic study with live sầu cells, dissection + + + 14025
Dissection, microscopic study with live cells + + 10X: 14065
Cell culture, tissue dissociation + + + + 24020 24010
+ + + 10X: 14060

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